diff --git a/Assets/YooAsset/Runtime/ResourcePackage/ManifestTools.cs b/Assets/YooAsset/Runtime/ResourcePackage/ManifestTools.cs
index 8d239c64..8b146f63 100644
--- a/Assets/YooAsset/Runtime/ResourcePackage/ManifestTools.cs
+++ b/Assets/YooAsset/Runtime/ResourcePackage/ManifestTools.cs
@@ -221,9 +221,16 @@ namespace YooAsset
             manifest.AssetDic = new Dictionary<string, PackageAsset>(assetCount);
 
             if (manifest.EnableAddressable)
+            {
                 manifest.AssetPathMapping1 = new Dictionary<string, string>(assetCount * 3);
+            }
             else
-                manifest.AssetPathMapping1 = new Dictionary<string, string>(assetCount * 2);
+            {
+                if (manifest.LocationToLower)
+                    manifest.AssetPathMapping1 = new Dictionary<string, string>(assetCount * 2, StringComparer.OrdinalIgnoreCase);
+                else
+                    manifest.AssetPathMapping1 = new Dictionary<string, string>(assetCount * 2);
+            }
 
             if (manifest.IncludeAssetGUID)
                 manifest.AssetPathMapping2 = new Dictionary<string, string>(assetCount);
@@ -245,8 +252,6 @@ namespace YooAsset
             // 填充AssetPathMapping1
             {
                 string location = packageAsset.AssetPath;
-                if (manifest.LocationToLower)
-                    location = location.ToLower();
 
                 // 添加原生路径的映射
                 if (manifest.AssetPathMapping1.ContainsKey(location))
diff --git a/Assets/YooAsset/Runtime/ResourcePackage/PackageManifest.cs b/Assets/YooAsset/Runtime/ResourcePackage/PackageManifest.cs
index 0640d284..8942a865 100644
--- a/Assets/YooAsset/Runtime/ResourcePackage/PackageManifest.cs
+++ b/Assets/YooAsset/Runtime/ResourcePackage/PackageManifest.cs
@@ -138,9 +138,6 @@ namespace YooAsset
             if (string.IsNullOrEmpty(location))
                 return string.Empty;
 
-            if (LocationToLower)
-                location = location.ToLower();
-
             if (AssetPathMapping1.TryGetValue(location, out string assetPath))
                 return assetPath;
             else
@@ -307,9 +304,6 @@ namespace YooAsset
                 return string.Empty;
             }
 
-            if (LocationToLower)
-                location = location.ToLower();
-
             if (AssetPathMapping1.TryGetValue(location, out string assetPath))
             {
                 return assetPath;