diff --git a/Assets/YooAsset/Runtime/DiagnosticSystem/RemoteDebuggerInRuntime.cs b/Assets/YooAsset/Runtime/DiagnosticSystem/RemoteDebuggerInRuntime.cs
index b41c5485..2c8b5d7b 100644
--- a/Assets/YooAsset/Runtime/DiagnosticSystem/RemoteDebuggerInRuntime.cs
+++ b/Assets/YooAsset/Runtime/DiagnosticSystem/RemoteDebuggerInRuntime.cs
@@ -7,6 +7,15 @@ namespace YooAsset
 {
     internal class RemoteDebuggerInRuntime : MonoBehaviour
     {
+#if UNITY_EDITOR
+        [RuntimeInitializeOnLoadMethod(RuntimeInitializeLoadType.SubsystemRegistration)]
+        private static void OnRuntimeInitialize()
+        {
+            _sampleOnce = false;
+            _autoSample = false;
+        }
+#endif
+
         private static bool _sampleOnce = false;
         private static bool _autoSample = false;
 
diff --git a/Assets/YooAsset/Runtime/DiagnosticSystem/RemoteEditorConnection.cs b/Assets/YooAsset/Runtime/DiagnosticSystem/RemoteEditorConnection.cs
index fe388a59..9b8cbb53 100644
--- a/Assets/YooAsset/Runtime/DiagnosticSystem/RemoteEditorConnection.cs
+++ b/Assets/YooAsset/Runtime/DiagnosticSystem/RemoteEditorConnection.cs
@@ -8,6 +8,14 @@ namespace YooAsset
 {
     internal class RemoteEditorConnection
     {
+#if UNITY_EDITOR
+        [RuntimeInitializeOnLoadMethod(RuntimeInitializeLoadType.SubsystemRegistration)]
+        private static void OnRuntimeInitialize()
+        {
+            _instance = null;
+        }
+#endif
+
         private static RemoteEditorConnection _instance;
         public static RemoteEditorConnection Instance
         {
diff --git a/Assets/YooAsset/Runtime/DiagnosticSystem/RemotePlayerConnection.cs b/Assets/YooAsset/Runtime/DiagnosticSystem/RemotePlayerConnection.cs
index 05f8db53..5522bda9 100644
--- a/Assets/YooAsset/Runtime/DiagnosticSystem/RemotePlayerConnection.cs
+++ b/Assets/YooAsset/Runtime/DiagnosticSystem/RemotePlayerConnection.cs
@@ -8,6 +8,14 @@ namespace YooAsset
 {
     internal class RemotePlayerConnection
     {
+#if UNITY_EDITOR
+        [RuntimeInitializeOnLoadMethod(RuntimeInitializeLoadType.SubsystemRegistration)]
+        private static void OnRuntimeInitialize()
+        {
+            _instance = null;
+        }
+#endif
+
         private static RemotePlayerConnection _instance;
         public static RemotePlayerConnection Instance
         {
diff --git a/Assets/YooAsset/Runtime/DownloadSystem/DownloadSystemHelper.cs b/Assets/YooAsset/Runtime/DownloadSystem/DownloadSystemHelper.cs
index bd1f24e8..f5ccfa3e 100644
--- a/Assets/YooAsset/Runtime/DownloadSystem/DownloadSystemHelper.cs
+++ b/Assets/YooAsset/Runtime/DownloadSystem/DownloadSystemHelper.cs
@@ -1,4 +1,5 @@
 using UnityEngine.Networking;
+using UnityEngine;
 
 namespace YooAsset
 {
@@ -9,6 +10,14 @@ namespace YooAsset
 
     internal class DownloadSystemHelper
     {
+#if UNITY_EDITOR
+        [RuntimeInitializeOnLoadMethod(RuntimeInitializeLoadType.SubsystemRegistration)]
+        private static void OnRuntimeInitialize()
+        {
+            UnityWebRequestCreater = null;
+        }
+#endif
+
         public static UnityWebRequestDelegate UnityWebRequestCreater = null;
         public static UnityWebRequest NewUnityWebRequestGet(string requestURL)
         {
diff --git a/Assets/YooAsset/Runtime/DownloadSystem/WebRequestCounter.cs b/Assets/YooAsset/Runtime/DownloadSystem/WebRequestCounter.cs
index 0c962d91..72984b55 100644
--- a/Assets/YooAsset/Runtime/DownloadSystem/WebRequestCounter.cs
+++ b/Assets/YooAsset/Runtime/DownloadSystem/WebRequestCounter.cs
@@ -6,6 +6,14 @@ namespace YooAsset
 {
     internal class WebRequestCounter
     {
+#if UNITY_EDITOR
+        [UnityEngine.RuntimeInitializeOnLoadMethod(UnityEngine.RuntimeInitializeLoadType.SubsystemRegistration)]
+        private static void OnRuntimeInitialize()
+        {
+            _requestFailedRecorder.Clear();
+        }
+#endif
+
         /// <summary>
         /// 记录网络请求失败事件的次数
         /// </summary>
diff --git a/Assets/YooAsset/Runtime/OperationSystem/OperationSystem.cs b/Assets/YooAsset/Runtime/OperationSystem/OperationSystem.cs
index 74d5fe6b..75a22a21 100644
--- a/Assets/YooAsset/Runtime/OperationSystem/OperationSystem.cs
+++ b/Assets/YooAsset/Runtime/OperationSystem/OperationSystem.cs
@@ -6,6 +6,14 @@ namespace YooAsset
 {
     internal class OperationSystem
     {
+#if UNITY_EDITOR
+        [UnityEngine.RuntimeInitializeOnLoadMethod(UnityEngine.RuntimeInitializeLoadType.SubsystemRegistration)]
+        private static void OnRuntimeInitialize()
+        {
+            DestroyAll();
+        }
+#endif
+
         private static readonly List<AsyncOperationBase> _operations = new List<AsyncOperationBase>(1000);
         private static readonly List<AsyncOperationBase> _newList = new List<AsyncOperationBase>(1000);
 
diff --git a/Assets/YooAsset/Runtime/Settings/YooAssetSettingsData.cs b/Assets/YooAsset/Runtime/Settings/YooAssetSettingsData.cs
index 13ce8b80..6ea1560b 100644
--- a/Assets/YooAsset/Runtime/Settings/YooAssetSettingsData.cs
+++ b/Assets/YooAsset/Runtime/Settings/YooAssetSettingsData.cs
@@ -5,6 +5,14 @@ namespace YooAsset
 {
     public static class YooAssetSettingsData
     {
+#if UNITY_EDITOR
+        [UnityEngine.RuntimeInitializeOnLoadMethod(UnityEngine.RuntimeInitializeLoadType.SubsystemRegistration)]
+        private static void OnRuntimeInitialize()
+        {
+            _setting = null;
+        }
+#endif
+
         private static YooAssetSettings _setting = null;
         internal static YooAssetSettings Setting
         {
diff --git a/Assets/YooAsset/Runtime/YooAssets.cs b/Assets/YooAsset/Runtime/YooAssets.cs
index 4a112c42..5dc8a2a6 100644
--- a/Assets/YooAsset/Runtime/YooAssets.cs
+++ b/Assets/YooAsset/Runtime/YooAssets.cs
@@ -9,6 +9,16 @@ namespace YooAsset
 {
     public static partial class YooAssets
     {
+#if UNITY_EDITOR
+        [RuntimeInitializeOnLoadMethod(RuntimeInitializeLoadType.SubsystemRegistration)]
+        private static void OnRuntimeInitialize()
+        {
+            _isInitialize = false;
+            _packages.Clear();
+            _defaultPackage = null;
+        }
+#endif
+
         private static bool _isInitialize = false;
         private static GameObject _driver = null;
         private static readonly List<ResourcePackage> _packages = new List<ResourcePackage>();
diff --git a/Assets/YooAsset/Runtime/YooAssetsDriver.cs b/Assets/YooAsset/Runtime/YooAssetsDriver.cs
index 176104f8..d576bfcb 100644
--- a/Assets/YooAsset/Runtime/YooAssetsDriver.cs
+++ b/Assets/YooAsset/Runtime/YooAssetsDriver.cs
@@ -5,6 +5,14 @@ namespace YooAsset
 {
     internal class YooAssetsDriver : MonoBehaviour
     {
+#if UNITY_EDITOR
+        [RuntimeInitializeOnLoadMethod(RuntimeInitializeLoadType.SubsystemRegistration)]
+        private static void OnRuntimeInitialize()
+        {
+            LastestUpdateFrame = 0;
+        }
+#endif
+
         private static int LastestUpdateFrame = 0;
 
         void Update()